NMR Advanced Training Info
- Training for access to the NMR Spectrometers in the NMR Core is geared toward people who are in research groups and generating their own samples. Everyone goes through some kind of hands-on assessment of their ability to operate a spectrometer. This includes under-graduates, graduate students, “zero year” graduate students, post-docs, faculty and visitors. All users must have an iLab account with an associated funding source and chart field.
- “Drop-off NMR” offers a hands-off approach to obtaining NMR data. A brief training maybe required to show the user how to navigate the software (“IconNMR”) and load samples on the sample changer. Samples are run in auto-mation Monday through Friday nights on the B600 spectrometer. Sample information is submitted on a web form and clearly labeled samples should be placed on the NMR tube rack outside of CA051 by 5pm each day. File names must only contain letters, numbers, underscores and hyphens – NO spaces, commas or other punctuation. IconNMR will save data on the computer and email a compressed data folder for each successful run. Sample(s) should be picked up the next day. Samples left for a week or so will be moved to the hood in the Sample Prep Lab in CA051.
- If you would like to run your own NMR experiments and at time other than overnight, you can request general training on the “Request Services” tab in the NMR Lab on iLab. The first step in training includes a virtual session covering basic procedures to acquire NMR data, the NMR spectrometers in the core, and using the NEO400 and NEO600 NMR Spectrometers which run in automation 24/7. The NEO400 has a nitrogen cooled broadband cryoprobe that runs many nuclei with good sensitivity. The NEO600 has a helium cooled cryoprobe optimized for 13C and 31P and very good 1H sensitivity – second best in the NMR Core.
- For access to the 400NB, 500 and B600 spectrometers, one must run Topspin, Bruker’s NMR software, directly. Individuals schedule this training on the 500 or B600 by submitting a request in iLab. The 400NB tends to be used for routine 1H and 19F spectra, the 500 for variable temperature work, longer runs (think reaction kinetics and dynamics), and photo- NMR, which are all covered in a specific training. The B600 is equipped with a helium cooled cryoprobe optimized for 1H (most sensitivity in the core) and 19F and capable of high sensitivity 13C spectra.
- Individual training sessions are scheduled for specific experiments, like variable temperature, DOSY, relaxation, etc. NMR Staff will coordinate time for direct one-on-one training on a spectrometer once requested through the “Request Services” tab in iLab.
- “Advanced NMR Training” comprises multiple virtual and hands-on opportunities to fine tune your NMR skills to obtain high quality NMR data. Topics include advanced shimming methods, navigating 2D acquisition para-meters, speeding up 2D experiments with Non-Uniform Sampling (‘NUS’) and a discussion of nuclear spin relaxation. These sessions are scheduled intermittently through out the year.